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«Taxonomic Revision, Molecular Phylogeny and Zoogeography of the huntsman spider genus Eusparassus (Araneae: Sparassidae) Dissertation for attaining ...»

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Results: chapter 3.3: Molecular phylogeny

Results: chapter 3.3: Molecular phylogeny

Table 1. List of voucher specimens exclusively treated for this study, with respective voucher numbers (SD: Spider DNA collection number in SMF), collection localities and GenBank accession numbers. All species belongs to family Sparassidae except noted. Sparassidae species mentioned first and in alphabetical order.

–  –  –

Table 2. List of specimen’s included in the phylogenetic analyses and respective accession numbers extracted from GenBank (http://www.

ncbi.nlm.nih.gov/nuccore). The higher level classification follows Dunlop and Penny (2011).

–  –  –

Table 3. Molecular markers and primers used for amplification.

The amplification was performed in 25 µl final volume containing 13.95 µl of ultra-pure water (dd H2O), 2.5 µl of 10*Polymerase-buffer, 0.4 µl of each primer (100 pmol/µl), 1.5 µl of dNTPs (2.5 mM), 3.5 µl of MgCl2 (25 mM), 2.5 µl of the genomic spider DNA templates (30–35 ng/ µl) and 0.25 µl of Taq DNA polymerase. PCR settings list initial denaturation (id; indicating temperature in °C followed by time in seconds) followed by *n cycles (denaturation: d, anneiling: a, elongation: e) in [], and a terminal elongation (te). References: 1. Bayer and Schönhofer (2013); 2. Bayer (2008; both based on Barrett & Hebert, 2005); 3. based on Simon et al.

(1994) modified for this study; 4. based on Crews and Hedin (2006) modified for this study; 5. Hedin and Maddison (2001); 6. Colgan et al. (1998).

–  –  –

Table 4. Models of evolution for each partition and different sets of taxon combinations as calculated by jModeltest.

Alignment length did not vary for COI (603bp) and H3 (327bp).

–  –  –

4. DISCUSSION In this chapter I am summarising and linking the three chapters of the results

containing two publications (chapter 3.1: Moradmand and Jäger 2012a; chapter 3.2:

Moradmand 2013) and one manuscript ―under review‖ (chapter 3.3). I am focusing here on the main results and highlighting the achievements of my study.

4.1. Taxonomic revision and systematics of Eusparassus based on morphology It is essential to any kind of biological research (i.e. performing a study in phylogeny and zoogeography of a group of organisms) to have clearly defined units (species taxon). Prior to this study, the genus Eusparassus was not revised and composed of a heterogeneous assembly of several misplaced taxa, some descriptions solely based on a single sex or juveniles and several with nomenclatural ambiguities.

Moreover many species were new to science (~27%) and had to be described.

Eusparassus demonstrates a roughly uniform morphology which hinders the recognition of its diversity (Levy 1989). I have carried out three main steps to revise

the genus:



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