WWW.DISSERTATION.XLIBX.INFO
FREE ELECTRONIC LIBRARY - Dissertations, online materials
 
<< HOME
CONTACTS



Pages:     | 1 |   ...   | 34 | 35 || 37 | 38 |   ...   | 112 |

«Strengthening the Nation through Diversity, Innovation & Leadership in STEM San Antonio,Texas · October 3-6, 2013 Get Connected! Connect with the ...»

-- [ Page 36 ] --

Natural products are complex organic molecules made by a variety of organisms including bacteria, fungi, and plants which often possess bioactivities that make them useful as drugs, drug leads, or probes for deciphering biological networks. Using bioinformatics tools developed in the Melançon lab and elsewhere, we have identified and partially annotated 6 natural product gene clusters of interest in Actinosynnema mirum, including 2 encoding analogs of ansamycin class anticancer compounds. To determine if these 6 gene clusters are transcriptionally active, we have designed and begun to optimize real-time PCR assays targeting 1 to 2 genes in each cluster by creating four cDNA libraries from mRNA samples collected at time points representing several growth phases of A. mirum. From this, we have determined that 2 of the 6 gene clusters of interest, including 1 of the clusters encoding an ansamycin analog, are transcriptionally active. Additionally, we have also cultured A. mirum in large scale and have obtained crude natural product extracts from it using standard isolation techniques, including organic extraction and adsorption onto Amberlite XAD-7 and XAD-16 resins. Extracts will be analyzed for the predicted compound using analytical HPLCESI mass spectrometry. Once compounds of interest are identified, they will be purified using preparative HPLC.

The effect of the ansamitocin analog will be assessed in an in vitro assay. If active, its structure will be determined by NMR. Other purified compounds will also be assessed for antibacterial and anticancer activities by our group and by collaborators.

SAT-563

CULTIVATION OF HETEROTROPHIC MARINE BACTERIA ALONG THE PACIFIC COAST OF NORTH AMERICA

Maria Ortiz, Erin Schaadt, Lizzette Rojas, and Jesse Dillon.

California State University, Long Beach, Long Beach, CA.

Environmental factors vary geographically along coasts (i.e., temperature) and may affect the abundance and diversity of marine heterotrophic bacteria along the Pacific coast. This project involved isolation of cultures from seawater samples collected from sandy beaches from Baja California to northern Oregon in January and August of 2010. Monthly samples were collected at Huntington Beach. It was hypothesized that heterotrophic marine bacteria would display significant differences in diversity and abundance geographically (north and south of Point Conception) as well as seasonally (summer and winter). Bacteria were counted via serial dilution (MPNs) in marine broth to determine abundance. Cultures were isolated via streak plate serial dilution and were characterized by Gram staining and light microscopy. Nucleic acid extractions, PCR amplification, and sequencing of 16S rRNA genes were performed to construct a neighbor joining tree using ARB software. In parallel, salinity tolerance experiments were done on marine broth isolates (N = 77) using treatments of 3.5%, 15%, and 23% salinity. There was no significant difference in bacterial abundance between seasons after comparison of winter and summer MPNs (unpaired t-test, p = 0.057). Within the seasons, there were no strong relationships between temperature and bacterial abundance (winter R2 = 0.005, summer R2 = 0.127). The salinity experiment showed growth only in the 3.5% concentration.

Sequencing analysis revealed diverse assemblages of Gamaproteobacteria, Flavobacteria, Actinobacteria, Alphaproteobacteria, and Bacillus spp. along coastal North America. Current phylogenetic analyses indicate biogeographical influence south of Point Conception along with seasonality of Actinobacteria and species of Vibrio and Psychrobacter.

118 UNDERGRADUATE POSTER ABSTRACTS

Biological Sciences SAT-573

COMPARISON OF MATRIX COMPONENT AND DRUG SUSCEPTIBILITY BETWEEN CANDIDA ALBICANS AND

NON-ALBICANS SPECIES

Keely Redhage, Hiram Sanchez, David Andea.

University of Wisconsin, Madison, Madison, WI.

Fungal infections are becoming an increasing problem in hospital settings leading to growing mortality rates and skyrocketing health-care costs. The most prominent fungal infection is due to Candida albicans and non-albicans species Candida glabrata, Candida parapsilosis, and Candida tropicalis. These pathogens, when attached to a surface such as a catheter, produce a biofilm which is highly resistant to antifungal drugs. Most of the drug resistance is due to the extracellular material (matrix) secreted by the biofilm. This study focuses on matrix components of nonalbicans species in comparison to C. albicans. Our hypothesis is that the matrix components of biofilms are key to antifungal resistance. Carbohydrates such, β-1,3/1,6 glucan, mannans, and proteins are present in matrix. In order to understand how these components contribute to drug resistance, matrix was produced by growing the organisms in roller bottles forming a biofilm, and the matrix was harvested after 48 hours. Samples were dialyzed and lyophilized.

The matrix components were analyzed with phenol sulfuric assay (for total carbohydrate content), BCA assay (for total protein content), and ELISAs for mannans and β-1,3/1,6 glucan (for specific carbohydrate content). Although all components of the matrix play a role in antifungal resistance, preliminary data show that carbohydrates are the most important components in the drug resistance mechanism. This was shown by XTT assays with a drug combination of tunicamycin and fluconazole where a correlation was found between the amount of matrix carbohydrates and drug susceptibility between strains.





FRI-578

ASPERGILLUS MOLD – BACTERIA COCULTURE: IMPROVED POLYCYCLIC AROMATIC HYDROCARBON

DEGRADATION THROUGH SYNERGISTIC METABOLISM

Marissa Hartleb1, Mi-kyung Lee2.

Cornell University, Ithaca, NY, 2University of Wisconsin-Madison, Madison, WI.

1 Tens of thousands of Superfund sites, abandoned or inactive hazardous waste sites recognized by the Environmental Protection Agency (EPA), are found throughout the United States and hold dangerous contaminants such as polycyclic aromatic hydrocarbons (PAHs). PAHs are carcinogenic, mutagenic, and highly toxic and also have high persistence in soil. Some organisms have been found in Superfund sites that can metabolize these contaminants.

Bacterial organisms have been found which degrade PAHs with 2 to 3 benzene rings, while fungal organisms have been found which degrade PAHs with 4 or more rings. The hypothesis for this experiment is that by having the 2 types of organisms live and work together, the range and quality of PAH degradation will increase. This idea will be tested using a Transwell-24 system. This system will allow small metabolites to be exchanged between the two organisms but will disallow all physical interaction. The fungus Aspergillus nidulans is a common soil fungus known to degrade benzo[a]pyrene, a 5-ring PAH. The bacterium Burkholderia sp. Ch1-1, is found in Superfund sites and is known to degrade phenanthrene, a 3-ring PAH. It is suspected that the fungus will mineralize pyrene, a 4-ring PAH, which will then allow the bacterium to metabolize pyrene. If the hypothesis is correct, improved bioremediation strategies will become possible, as well as more accurate PAH biosensors which can be used to measure initial concern or subsequent success.

FRI-582

ANALYSIS OF GROWTH PATTERNS OF PSEUDOMONAS STUTZERI AND STREPTOCOCCUS EPIDERMIDIS: A

MULTISPECIES BIOFILM

Nnenna Kpando, Lea Means, Kimberly Yawn, Caroline Barbosa, Antonio Mendoza, Poonam Gulati, Youn-Sha Chan.

University of Houston-Downtown, Houston, TX.

The objective of this project is to analyze the growth and development patterns of bacteria living in communities known as biofilms. A biofilm is a communal of single-species or multiple-species microorganisms that adhere to a moist surface and to one another using extra polymeric substances that are synthesized by them. Bacteria can also have an independent growth pattern known as planktonic or free-swimming. The growth pattern of planktonic cells is well studied and shows a logistic growth pattern. Studies in our lab on single species biofilms of Streptococcus epidermidis, a Gram-positive, coccus-shaped bacterium, and Pseudomonas stutzeri, a Gram-negative, rod-shaped bacterium, have demonstrated a cyclic pattern of growth. We have developed a mathematical model for the cyclic growth pattern. This model, which is a Bessel function, predicts the cyclic nature of the growth and a dampening

119 UNDERGRADUATE POSTER ABSTRACTS

of the cycles with time. In nature, multispecies biofilms are commonly found. As such, in this project, the multiplespecies biofilm growth patterns of S. epidermidis and P. stutzeri are being studied as a mixed species biofilm. Based on our studies of the two bacteria, we predict that as resources deplete, P. stutzeri will overshadow and outlast S.

epidermidis. We plan to develop a predator-prey model between the two organisms that establishes growth peaks for one organism and troughs for the other over a period of time. The model could then be used to explain some of the dynamics of natural biofilms.

SAT-568

IDENTIFICATION OF BACTERIA OF THE RIO GRANDE WATERS BY DGGE ANALYSIS

Melissa Saucedo1, Rebecca Castro1, Brittan Wilson2, and Monica Mendez1.

Texas A&M International University, Laredo, TX, 2University of Baltimore, Baltimore, MD.

1 Previous studies examining the microbial community of the Rio Grande border have focused on culturable pathogenic bacteria, mainly E.coli. This study will focus on the identification of noncultured bacteria obtained from a previous study using denaturing gradient gel electrophoresis (DGGE) of the 16S rRNA gene amplified from the DNA extracts of three water sampling sites within the Laredo, Texas, and Nuevo Laredo, Mexico, border of the Rio Grande. The three sampling sites were Zacate Creek area (Site 1), International Bridge I (Site 2), and the Jefferson Water Treatment Plant (Site 3). Sites were chosen based on effluent discharge points that were greatest at Site 1. Phylotype bands were excised from a DGGE gel after DGGE-PCR using primers 1070F and 1406-GC. Eluted DNA from each band will be reamplified using DGGE-PCR followed by cloning using the TOPO-TA cloning kit. Plasmids will then be purified for sequencing using T3 and T7 primers. Sequences will then be used for bacterial identification using the NCBI-BLAST database. Although all sites were similar (80%, Dice coefficient) based on DGGE profiles, Site 1 contained the most unique types of bacteria and was significantly greater in heterotrophic bacterial counts (9.4 x 103 CFU/ml, p 0.01) than all other sites. We expect that Site 1 will have an even greater number of unique phylotypes identified assuming that the DGGE bands will contain a greater number of mixed templates. This study will allow us to identify potential microbial biomarkers for understanding fluxes of nutrients and contaminants in the Rio Grande waters.

SAT-579

DISCOVERING THE STRUCTURE OF AN IMPORTANT HUMAN HEALTH ENZYME IN ENTEROCOCCUS

FAECIUM Amber Anderson, Gilbert John.

Oklahoma State University, Stillwater, OK.

Enterococcus faecium is an anaerobic bacterium that flourishes within the human intestine. This organism contains an important enzyme called azoreductase (AzoM), which is involved in cleaving the azo bond within azo dyes, producing a toxic, aromatic amine. Carcinogenic compounds can result from the reduction of dyes, which are commonly used to color food, clothing, drugs, and cosmetics. In terms of structure and function of the enzyme, very few intestinal bacteria containing azoreductase have been studied. This study used sophisticated crystallization methods to obtain the structure of AzoM. The pure AzoM protein was obtained from an Escherichia coli expression system, extracted using BugBuster Protein Extraction Kit, and protein purity was verified by SDS-PAGE gel electrophoresis. The protein sample was collected in two different buffer conditions, Tris and HEPES, and concentrated to over 10 mg/ ml. Initial protein crystallization was obtained by the sitting drop method using the 96-well Hamilton and Index Crystal screens. Positive samples were then optimized using additive screens as well as 24-well, custom-prepared conditions based on varying concentrations of salts, dyes, buffers, and pHs. Based on microscopic examination, crystals with sufficient size were subjected to X-ray crystallography using the Brookhaven National Lab, National Synchrotron Light Source. Computer software was used to create the 3D structure based on data from X-ray diffraction. Analysis of crystallization will provide an understanding of the structure and function of AzoM, ultimately impacting the environmental, medical, and biotechnology fields.

FRI-571

ISOLATION AND PURIFICATION OF AN ACTIVE COMPOUND PRODUCED BY STREPTOMYCES BACILLARIS

AGAINST STAPHYLOCOCCUS AUREUS

Melisa Fuentes, Luis Mota-Bravo.

University of California, Irvine, Irvine, CA.

The emergence of multidrug resistance in Staphylococcus aureus has triggered an urgent need for new antibiotics and novel antibiotic combinations against both Gram-positive and Gram-negative bacteria. Actinobacteria, in

120 UNDERGRADUATE POSTER ABSTRACTS

Biological Sciences particular, the genus Streptomyces, produce almost two-thirds of today’s clinically useful antibiotics. The objective of this study is to isolate actinobacteria from local soil samples and investigate their potential as producers of antibiotics against Staphylococcus aureus by using direct overlay assays with S. aureus ATCC 25923. A neomycin producer, Streptomyces fradiae ATCC 10745, was used as a positive control. Inhibition zones surrounding actinobacteria colonies were measured after overnight incubation at 35 oC. Active compounds were extracted using ethyl acetate.

The extracts were separated by thin-layer chromatography (TLC) using different ratios of methanol-dichloromethane.



Pages:     | 1 |   ...   | 34 | 35 || 37 | 38 |   ...   | 112 |


Similar works:

«Haydn and Eighteenth-Century Patronage in Austria and Hungary H. C. ROBBINS LANDON THE TANNER LECTURES HUMAN VALUES ON Delivered at Clare Hall, Cambridge University February 25 and 26, 1983 H. C. ROBBINS LANDON was educated at Swarthmore College and Boston University. H e founded the Haydn Society in Boston in 1949 and subsequently settled in Vienna, where he became a member of the Zentralinstitut fur Mozartforschung (Salzburg), a contributor to the British Broadcasting Corporation, and a...»

«Committee on the Study of Religion PhD Student CVs November 2015 Timothy M. Baker 1 Eleanor Craig_ 5 Aaron Goldman 9 Filipe Maia_ 12 Lina Verchery_ 15 Kirsten M.Y. Wesselhoeft 20 Klaus C. Yoder 23 TIMOTHY M. BAKER 576 Cream Street, Thetford Center, Vermont, 05075 Cell: (716) 866-7743 scholar.harvard.edu/tmbaker tbaker@mail.harvard.edu EDUCATION: 2015 Doctor of Theology, Harvard Divinity School: Cambridge, Massachusetts Dissertation: “‘Be You as Living Stones Built Up, A Spiritual House, A...»

«Mark Scheme (Results) Summer 2013 GCE Geography (6GE01) Paper 01 Global Challenges Edexcel and BTEC Qualifications Edexcel and BTEC qualifications come from Pearson, the world’s leading learning company. We provide a wide range of qualifications including academic, vocational, occupational and specific programmes for employers. For further information visit our qualifications websites at www.edexcel.com or www.btec.co.uk for our BTEC qualifications. Alternatively, you can get in touch with us...»

«An Experiment In Misery Stephen Crane Author : Short Stories Category : dsmith0542 November 2009 Submit by : Read this on Full Online Books Link : www.fullonlinebooks.com Source : It was late at night, and a fine rain was swirling softly down, causing the pavements to glisten with hue of steel and blue and yellow in the rays of the innumerable lights. A youth was trudging slowly, without enthusiasm, with his hands buried deep in his trousers' pockets, toward the downtown places where beds can...»

«Un Tríptico de la Flagelación con San Benito y San Bernardo del taller de Jan van Dornicke en el Museo de Bellas Artes de Bilbao Ana Diéguez Rodríguez Este texto se publica bajo licencia Creative Commons del tipo reconocimiento–no comercial–sin obra derivada (by-nc-nd) 4.0 international. Puede, por tanto, ser distribuido, copiado y reproducido (sin alteraciones en su contenido), siempre con fines docentes o de investigación, y reconociendo su autoría y procedencia.No está permitido...»

«Studying Social Studies: Using Personal Narratives to Explore the Shifting Social Studies Curriculum Item type text; Electronic Thesis Authors Tejeda, Victoria Alexandria Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission...»

«Strengthened by Grace for a Life of Love “This book is a breath of fresh air, not because it’s personal (which it is), or because it’s practical (which it is), but because it is profoundly biblical. We found Gloria’s Scripture-saturated counsel to be eminently realistic and deeply encouraging. Her wit and wisdom will be good for the pastor and good for the pastor’s wife, which is good news for those in ministry and good news for the church.” Kevin and Trisha DeYoung, Senior Pastor,...»

«Press Contact: Bridget Morgan Contact Info for Publication: bmorgan@5thavenue.org 206-625-1900 / 888-5TH-4TIX (584-4849) (206) 260-2174 www.5thavenue.org Full Casting Announced for a Thrilling New ‘Revisal’ of Lerner & Loewe’s Paint Your Wagon at The 5th Avenue Theatre Featuring some of the most exhilarating songs ever written for the stage, this mythic Western is reborn with a new book adaptation by Pulitzer Prize nominee Jon Marans June 2-25, 2016 Music by Frederick Loewe; Book and...»

«Ensurepass.com Easy Test! Easy Pass! Microsoft MTA 98-364 Exam Vendor:Microsoft Exam Code: 98-364 Exam Name: Database Administration Fundamentals Download the complete collection of Exam's Real Q&As www.ensurepass.com Ensurepass.com Easy Test! Easy Pass! QUESTION 1 John works as a Database Administrator for Bluewell Inc. The company has a SQL Server database. A table in the database has a candidate key and an attribute that is not a constituent of the candidate key. The non-key attribute...»

«Dr. Babasaheb Ambedkar Marathwada University, Aurangabad Syllabus Sociology M.A. First Year To be implemented from 2009-2010 Dr. Babasaheb Ambedkar Marathwada University, Aurangabad Revised Syllabus for Master of Arts in Sociology (Under the faculty of social sciences) 1) Year of implementation Will be implemented form June 2009 The pattern of examination will be Semester system 2) Pattern (30 marks semester examination and 20 marks internal evaluation 3) Admission Criteria Candidate who has...»

«Office of Federal Energy Milepost • Regulatory Commission Energy Projects * December 2014 Sabine Pass Liquefaction Expansion, LLC Sabine Pass Liquefaction, LLC, and Sabine Pass LNG, L.P. Docket No. CP13-552-000 Cheniere Creole Trail Pipeline, L.P. Docket No. CP13-553-000 Sabine Pass Liquefaction Expansion Project and Cheniere Creole Trail Pipeline Expansion Project Environmental Assessment Washington, DC 20426 Cooperating Agencies: United States Army Corps of Engineers United States...»

«Eleazer N. Klein Writer's E-mail Address 212.756.2376 Eleazer.Klein@srz.com June 24, 2016 VIA E-MAIL (IMSHAREHOLDERPROPOSALS@SEC.GOV) U.S. Securities and Exchange Commission Division of Investment Management Office of Disclosure and Review 100 F Street, N.E. Washington, DC 20549 Re: Exchange Act Rule 14a-8: Submission of Shareholder Proposal for the 2016 Proxy Statement of Franklin Limited Duration Income Trust Dear Sir or Madam: We are writing on behalf of Saba Capital Management, L.P. and...»





 
<<  HOME   |    CONTACTS
2016 www.dissertation.xlibx.info - Dissertations, online materials

Materials of this site are available for review, all rights belong to their respective owners.
If you do not agree with the fact that your material is placed on this site, please, email us, we will within 1-2 business days delete him.